Bogomoletz Institute of Physiology. National Academy of Sciences, Ukraine

 Department of General and Molecular Pathophysiology

 

 

 

Curriculum Vitae

Dr. Vasyl S. Nagibin

 

 

                                                                       

1. Contact information

 

Address:                                                        4 Bogomoletz St.,off. 330, Kiev 03150, Ukraine

 

Telephone:                                                    Work +380442562481

Home +380445228348

Mob. +380661974161

Fax:                                                               +380442562073

E-mail:                                                          This email address is being protected from spambots. You need JavaScript enabled to view it.

 

2. Personal information

 

Nationality:                                                   Ukrainian

Date of Birth:                                               25 Oct 1981

Civil status:                                                   Single

 

3. Current appointments

 

Research Assistant                           Department of General and Molecular Pathophysiology

 A.A. Bogomoletz Institute of Physiology 

 Ukrainian National Academy of Sciences

 Kiev, Ukraine

 

Scientific supervisor – Prof. A. Moibenko

Jun 2013 –

 

4. Previous appointments

 

Assistant                                            Department of Experimental Cardiology

 A.A. Bogomoletz Institute of Physiology

 Ukrainian National Academy of Sciences

 Kiev, Ukraine

 

Scientific supervisor – Prof. A. Moibenko

                                                          May 2002 – Oct 2004

 

PhD Student                                      Department of Experimental Cardiology

 A.A. Bogomoletz Institute of Physiology

 Ukrainian National Academy of Sciences

 Kiev, Ukraine

 

Scientific supervisor – Prof. A. Moibenko

                                                            Oct 2004 – Oct 2007

 

Research Assistant                           Department of General and Molecular Pathophysiology

 A.A. Bogomoletz Institute of Physiology 

 Ukrainian National Academy of Sciences

 Kiev, Ukraine

 

 Scientific supervisor – Prof. A. Moibenko

 Oct 2007 – Dec 2009

 Nov 2010 – Sep 2012

 

Postdoctoral position                        Department of Biochemistry, Biophysic and Biotechnology

 Jagellonian University

 Krakow, Poland

 

 Scientific supervisor – Dr.hub J.Cichy

                                                            Dec 2009 – Nov 2010

 

Postdoctoral position                         Institute of Heart Research

 Slovak Academy of Sciences

 Bratislava, Slovak

 

Scientific supervisor – Dr. N. Tribulova

Sep 2012 – June 2013

 

Lecturer                                             Department of Pathophysiology

                                                            National Medical University

                                                            Kiev, Ukraine

 

                                                           Supervisor  – Prof. N. Kryshtal

                                                            Sep 2005 –May 2009

 

5. Education/ Professional Qualifications

 

PhD (Pathological Physiology)        Department of Experimental Cardiology

 A.A. Bogomoletz Institute of Physiology

 Ukrainian National Academy of Sciences

 Kiev, Ukraine

 

Scientific supervisor – Prof. A. Moibenko

                                                            2004 – 2008

 

Medical Doctor

 

(General Medicine)                           National Medical University

                                                            Kiev, Ukraine, 1998 – 2004

 

6. Procedures and skills

 

  • Isolation and cultivation of different cells (neonatal cardiomyocytes, myofibroblasts, fibroblasts, neutrophiles, mast cells, macrophages etc)
  • Cell lines (MCF-7, fibroblasts)
  • Fluorescence microscopic techniques
  • Immunohisto(cyto)chemistry
  • Enzymes’ activity determination by hydrolysis of specific chromogenic and fluorogenic substrates (activities of proteasome, caspases, cathepsins, elastase, thrombin, plasmin, etc.)
  • Silencing of genes by si-RNA transfection, electroporation procedure
  • RNA, DNA isolation techniques
  • PCR, Real-time PCR
  • Western blot, Zymografy
  • Cytofluorimetry, FACS
  • Fractionation of cells and subcellular particles using Percoll, Ficoll
  • Animal handling experience (rabbits, rats, mice, including KO mice)
  • Animal surgery (modelling of coronary occlusion in rats)
  • Statistic analysis with use of software Statistica 5.0, Origin 7.0 etc.

 

7. Languages

 

English                       fluent

Ukrainian                   native

Russian                      native

 

 

 

8. IT skills

 

Confident with Microsoft Office (Word/Excel/PowerPoint/Outlook etc), Adobe Reader, Internet recourses and scientific databases  ( PubMed, OVID etc) and statistical analysis software (Statistica 5.0, Origin 7.0 etc).

 

9. Referees

 

Prof. Victor Dosenko. MD, PhD, Head of Department at General and molecular pathophysiology department, Bogomoletz Institute of Physiology, National academy of sciences of Ukraine

 

e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Dr. Olga V. Surova. PhD, Ludwig Institute for Cancer Research.
Oxygen Sensing and Cancer Group, Stockholm, Sweden.

 

e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

10. Publications

 

  • Veremeenko KN, Dosenko VE, Nagibin VS, Kizim AI, Moibenko AA. Proteolytic enzymes and apoptosis // Ukr. Biokhim. Zh.-2003.-Vol.75(6).-P.10-24.

 

  • Nagibin VS, Dosenko VE, Pyvovar SM, Moibenko OO, Iahupol's'kyi LM. Fluorine-containing analog of diazoxide prevented apoptosis in neonatal cardiomyocytes during anoxia-reoxygenation // Fiziol. Zh.-2004.-Vol.50(3).-P.3-8.

 

  • Dosenko V.E., Nagibin V.S., Moibenko A.A. Phosphocreatine (neoton) prevents necrotic and apoptotic death of cardiomyocytes in modelling of anoxia-reoxygenation // J. Mol. Cell. Cardiol.-2004.-Vol.36.-Issue 5.-P.746.

 

  • Tumanovs`ka L.V., Dosenko V.E., Nagibin V.S., Makogon N.V., Moibenko A.A. Apoptotic, autophagic and oncotic cardiomyocytes death in anoxia-reoxygenation // Fiziol. Zh.-2004.-Vol.50(5).-P.11-18.

 

§  Dosenko VE, Nagibin VS, Tumanovskaya LV, Zagoriy VY, Moibenko AA, Vaage J. Proteasome inhibitors eliminate protective effect of postconditioning in cultured neonatal cardiomyocytes. Fiziol Zh. 2006;52(3):15-24.

 

§  Dosenko VE, Nagibin VS, Tumanovska LV, Moibenko AA. Protective effect of autophagy in anoxia-reoxygenation of isolated cardiomyocyte? Autophagy. 2006 Oct-Dec;2(4):305-6. Epub 2006 Oct 25.

 

§  Dosenko VE, Zagorii VIu, Nagibin VS, Moibenko AA. Effect of N-3-methyladenine on peptidase and ribonuclease activity of proteasome Ukr Biokhim Zh. 2005 Sep-Oct;77(5):52-6.

 

§  Dosenko VE, Nagibin VS, Tumanovskaia LV, Zagorii VIu, Moibenko AA. The influence of quercetin on the activity of purified 20S, 26S proteasome and proteasomal activity in isolated cardiomyocytes Biomed Khim. 2006 Mar-Apr;52(2):138-45.

 

 §  Dosenko VE, Nagibin VS, Tumanovskaya LV, Zagoriy VY, Moibenko AA, Vaage J Proteasomal proteolysis in anoxia-reoxygenation, preconditioning and postconditioning of isolated cardiomyocytes. Pathophysiology. 2006 May;13(2):119-25.

 

§  Surovaya OV, Dosenko VE, Nagibin VS, Tumanovskaya LV, Moibenko AA. Effects of late postconditioning on gene expression and cell death in neonatal rat cardiomyocyte cultures. Pathophysiology. 2009 Jun;16(1):47-52.

 

§  Surova OV, Nagibin VS, Tumanovskaya LV, Dosenko VE, Moibenko AA. Effect of a low dose of proteasome inhibitor on cell death and gene expression in neonatal rat cardiomyocyte cultures exposed to anoxia-reoxygenation. Exp.Clin.Cardiol.2009;14(2):e57-61.

 

§  Lisovyy OO, Dosenko VE, Nagibin VS, Tumanovska LV, Korol MO, Surova OV, Moibenko OO.Cardioprotective effect of 5-lipoxygenase gene (ALOX5) silencing in ischemia-reperfusion. Acta Biochim Pol. 2009;56(4):687-94.

 

§  Pashevin DA, Tumanovska LV, Dosenko VE, Nagibin VS, Gurianova VL, Moibenko AA. Antiatherogenic effect of quercetin is mediated by proteasome inhibition in the aorta and circulating leukocytes. Pharmacol Rep. 2011;63(4):1009-18.

 

§  N Tribulova, V Nagibin, T Benova, C Viczenczova, J Radosinska, V Knezl, I Dovinova, M Barancik. Age-related deterioration of cardiac cell-to-cell coupling likely contributes to development of atrial fibrillation in old guinea pig. Cardiovascular Research, 2014; 103(suppl 1):S6.

 

§  Drevytska T., Nagibin V., Gurianova V., Moybenko O, Dosenko V. Silencing of TERT decreases levels of miR-1, miR-21, miR-29a and miR-208a in cardiomyocytes. Cell Biochem Funct. 2014;32(7):565-70.

 

§  Kyrychenko VO, Nagibin VS, Tumanovska LV, Pashevin DO, Gurianova VL, Moibenko AA, Dosenko VE, Klionsky DJ. Knockdown of PSMB7 induces autophagy in cardiomyocyte cultures: possible role in endoplasmic reticulum stress. Pathobiology. 2014; 81(1):8-14.

 

§  Drozdovska S., Gavenauskas B., Drevytska T., Nosar V., Nagibin V., Mankovska I., Dosenko V., and Kalinski M. SiRNA-induced silencing of hypoxia-inducible factor 3α increases endurance capacity in rats. Experimental Physiology (in press).

 

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