Bogomoletz Institute of Physiology. National Academy of Sciences, Ukraine

 Department of General and Molecular Pathophysiology

Georgii Portnichenko

 

Bogomolets street, 4, off. 326 Kyiv 01024, Ukraine

 

Home +38.044.256-24-81

E-mail: krissaegrim1@gmail.com

 

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OBJECTIVE: Researcher in fields of: pathophysiology, biochemistry, molecular physiology.

 

SUMMARY: The basic direction of scientific researches is study of sterol regulatory element-binding protein role in heart functioning under conditions of arterial hypertension and hypercholesterolemia

 

 

LAB SKILLS AND INSTRUMENTS:

·       RNA, DNA extraction using different methods/kits.

 

·       Performance of real-time PCR using Applied Biosystems® 7500 fast Real Time PCR

·       System and both CYBR Green and Taq-man technologies. Determination of both quantitative and relative expression levels, building a standard curve.

·       Gel electrophoresis.

·       Basic microscopy skills.

·       Evaluation of cardiac function parameters using Millar Instruments software

·       Measurements of enzyme reaction speed with spectrophotometer-based and titration based techniques.

·       Animal handling and basic animal surgery experience (mice, rats, pigs)

·       Evaluation of cardiac function parameters using Millar Instruments software

·       Images quantification methods

 

EXPERIENCE

 

Since 2012 assistant researcher of experimental cardiology department of O.O.Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine. Since 2010 PhD student of this department.

 

EDUCATION

 

At 2008 year graduated from biology department of Taras Shevchenko National University of Kyiv. PhD student of Institute of Cell Biology and Genetic Engineering, later PhD student of Department of General and Molecular Pathophysiology of O.O.Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine.

 

 

References Available Upon Request

 

 

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