Bogomoletz Institute of Physiology. National Academy of Sciences, Ukraine

 Department of General and Molecular Pathophysiology

Denis Pashevin

 

Contact information

 

Address:                                                        4 Bogomoletz St., off. 328, Kiev 03150, Ukraine

 

Telephone:                                                    Work +380442562481

Home +380442872808

Mob. +380965222963

Fax:                                                               +380442562073

E-mail:                                                          This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Personal information

 

Nationality:                                                   Ukrainian

Date of Birth:                                               26 Jan 1982

Civil status:                                                   Married

 

 Current appointments

 

Research Assistant                           Department of General and Molecular Pathophysiology

 A.A. Bogomoletz Institute of Physiology 

 Ukrainian National Academy of Sciences

 Kiev, Ukraine

 

Scientific supervisor – Prof. V. Dosenko

 

Lecturer                                             Department of Pathophysiology

                                                            National Medical University

                                                            Kiev, Ukraine

 

                                                           Supervisor  – Prof. N. Kryshtal

                                                            Sep 2004 –…

 

 Education/ Professional Qualifications

 

PhD (Pathological Physiology)        Department of Experimental Cardiology

 A.A. Bogomoletz Institute of Physiology

 Ukrainian National Academy of Sciences

 Kiev, Ukraine, National Medical University, Kiev, Ukraine

 

Scientific supervisor – Prof. A. Moibenko

                                                            2007 – 2010

 

Medical Doctor

 

(General Medicine)                           National Medical University

                                                            Kiev, Ukraine, 1998 – 2004

 

 Procedures and skills

 

  • Isolation and cultivation of different cells (neonatal cardiomyocytes, , neutrophiles, mast cells, macrophages etc)
  • Fluorescence microscopic techniques
  • Enzymes’ activity determination by hydrolysis of specific chromogenic and fluorogenic substrates (activities of proteasome, caspases, cathepsins, elastase, thrombin, plasmin, etc.)
  • Silencing of genes by si-RNA transfection, electroporation procedure
  • RNA, DNA isolation techniques
  • PCR, Real-time PCR
  • Fractionation of cells and subcellular particles using Percoll, Ficoll
  • Animal handling experience (rabbits, rats, mice, including KO mice)
  • Animal surgery (modelling of coronary occlusion in rats)
  • Statistic analysis with use of software Statistica 5.0, Origin 7.0 etc.

  

 IT skills

 

Confident with Microsoft Office (Word/Excel/PowerPoint/Outlook etc), Adobe Reader, Internet recourses and scientific databases  ( PubMed, OVID etc) and statistical analysis software (Statistica 5.0, Origin 7.0 etc).

 

 Referees

 

Prof. Victor Dosenko. MD, PhD, Head of Department at General and molecular pathophysiology department, Bogomoletz Institute of Physiology, National academy of sciences of Ukraine

 

e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

  

Publications

 

1.              D. O. Pashevin, V. E. Dosenko, L. V. Tumanovska, Yu. V. Bytz, O. O. Moibenko “Antiatherogenic Property of 'Corvitin': Effect on Proteasome Activity in Aorta, Heart and Blood Cells” // Int.J.Phys.Pathophys. – 2010 – Т.1 - №1 - С.35-43

2.              Pashevin DO, Dosenko VE, Nagibin VS, Moibenko OO.             «Antiatherogenic effect of quercetin is mediated by proteаsome inhibition in aorta and circulating leukocytes»// Pharmacological Reports – 2011 – Vol.4 – P. 954-968.

3.              Pashevin DO, Honcharov SV, Tumanovs'ka LV, Dosenko VIe, Moĭbenko OO. The changes in the activity of tripeptidyl peptidase II in experimental atherosclerosis and hypertension // Fiziol Zh. 2014;60(3):27-31.

4.              Honcharov SV, Portnichenko HV, Tumanovs'ka LV, Pashevin DO, Kuz'menko MO, Moĭbenko OO, Dosenko VIe. Effect of quercetin on proteasome activity in the aorta and heart tissues of spontaneously hypertensive rats // Fiziol Zh. 2014;60(3):3-10. Kyrychenko VO, Nagibin VS, Tumanovska LV, Pashevin DO, Gurianova VL, Moibenko AA, Dosenko VE, Klionsky DJ. Knockdown of PSMB7 induces autophagy in cardiomyocyte cultures: possible role in endoplasmic reticulum stress. Pathobiology. 2014;81(1):8-14.

5.              DO. Pashevin*, SV.Goncharov, LV. Tumanovska, VE. Dosenko, & O O. Moibenko.  Сhanges in Tripeptidyl Peptidase II Activity in Aortic Tissues under Experimental Atherosclerosis and Arterial Hypertension // International Journal of Physiology and Pathophysiology. 2015; 6 (2): 1-7

6.              "Proteasome Inhibition Diminishes Neutrophil Extracellular Traps Formation and Prevents Cardiomyocytes Death in Coculture with Activated Neutrophils During Anoxia-Reoxygenation" to "Pathobiology".

7.              Chernyshenko VO, Korolova DS, Dosenko VЕ, Pashevin DO, Kalchenko VI, et al // Calix[4]arene C-145 Effects on Plasma Haemostasis.// Pharm Anal Acta 2015; 6: 406. doi:10.4172/21532435.1000406

 

 

 

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