Bogomoletz Institute of Physiology. National Academy of Sciences, Ukraine

 Department of General and Molecular Pathophysiology

Our major research interest is to understand the molecular mechanisms of diseases, especially, of cardiovascular ones. We use experimental systems of different levels – from isolated organelles to whole organism, and a wide range of methods: registration of cardiohemodynamics, biochemical reactions, immunofluorescence and electron microscopy, RNA interference, qPCR, single-cell RT PCR, and many others. We use these approaches to understand cardiovascular pathology and to develop new therapeutic agents. Additionally, we perform population, functional, sports genetics, and pharmacogenetics studies. Our group of data scientists applies advanced methods of data analysis (MDR, random forest, and others) to get more valuable results from our experimental data. Our friendly team is open to collaboration!

<a href=Victor Dosenko" />

Victor Dosenko

Head of Department
MD PhD DrSc Professor

<a href=Angela Shysh" />

Angela Shysh

Group Leader
MSc PhD

<a href=Ruslan Strutynskyi" />

Ruslan Strutynskyi

Group Leader
MSc PhD

<a href=Tetiana Drevytska" />

Tetiana Drevytska

Senior Researcher
MSc PhD

<a href=Vasyl Nagybin" />

Vasyl Nagybin

Senior Researcher
MD PhD

<a href=Denis Pashevin" />

Denis Pashevin

Researcher
MD PhD

<a href=Dmytro Stroy" />

Dmytro Stroy

Researcher
MD PhD

<a href=Zoya Serebrovska" />

Zoya Serebrovska

Researcher
MSc, PhD

<a href=Anna Zhukovska" />

Anna Zhukovska

Researcher
MD PhD

<a href=Lesya Tumanovska" />

Lesya Tumanovska

Researcher
MSc

<a href=Oksana Klymenko" />

Oksana Klymenko

Researcher
MD PhD

<a href=Sergii Goncharov" />

Sergii Goncharov

Junior Researcher
MD

<a href=Tetiana Lapikova-Bryhinska" />

Tetiana Lapikova-Bryhinska

Junior Researcher
MSc

<a href=Anastasiya Maystrenko" />

Anastasiya Maystrenko

Junior Researcher
MSc

Roman Rovenets

Roman Rovenets

Scientific Engineer

Oleg Svarychevskiy

Oleg Svarychevskiy

Scientific Engineer

<a href=Georgii Portnichenko" />

Georgii Portnichenko

PhD Student
MSc

Ievgenii Gonchar

Ievgenii Gonchar

PhD Student

Michael Khetsuriani

Michael Khetsuriani

PhD student

<a href=Elizaveta Zhuravel" />

Elizaveta Zhuravel

PhD student
MD

 

Methods and techniques

 

Measurement of cardiac function using pressure–volume conductance catheter technique in mice and rats

Evaluation of hemostatic dressing in a groin puncture model in swine

The flexiVent is widely regarded as the gold standard for in vivo lung function measurements

Animal models of athero- and arteriosclerosis, arterial hypertension, myocardial infarction, hypetrophy of heart, diabetes mellitus, acid-base imbalance, denervation, acute hemorrhage, etc; fresh tumor cells inoculation (transplantaition) to rat, mice)

Restriction fragment length polymorphism analysis

Culture of neonatal cardiomyocytes

Western-blotting

Real-time PCR 

Electron and Fluorescence microscopic techniques (determination of apoptosis, necrosis, autophagy etc)

Single-cell reverse transcription real-time PCR

Enzymes’ activity determination using specific chromogenic and fluorogenic substrates (activities of proteasome, caspases, cathepsins, elastase, thrombin, plasmin, MMP, NOS, etc.)

RNA-interference

Immunohistochemical using specific monoclonal antibodies (immuno-fluorescent method) and specific fluorescent dyes.

Determination of histone acetylation status by chromatin immunoprecipitation

Fractionation of cells and subcellular particles using Percoll

 

Statistic analysis

 

Our Software

 

SNIP`ka is a specialized software developed and designed by Dmytro Stroy for statistical data analysis in science

 

Gurianova V, Stroy D, Kruzliak P, Kyrichenko V, Moibenko A, Dosenko V. Does proteasome regulate the level of microRNA-1 in cardiomyocytes? Application to anoxia-reoxygenation // Mol Cell Biochem. 2015 Jun;404(1-2):45-51.PubMed

Orlovsky MA, Dosenko VE, Spiga F, Skibo GG, Lightman SL.Hippocampus remodeling by chronic stress accompanied by GR, proteasome and caspase-3 overexpression. Brain Res. 2014 Dec 17;1593:83-94. PubMed

Drevytska TI, Nagibin VS, Gurianova VL, Kedlyan VR, Moibenko AA, Dosenko VE.Silencing of TERT decreases levels of miR-1, miR-21, miR-29a and miR-208a in cardiomyocytes. Cell Biochem Funct. 2014 Oct;32(7):565-70. PubMed

Drozdovska SB, Dosenko VE, Ahmetov II, Ilyin VN.The association of gene polymorphisms with athlete status in ukrainians. Biol Sport. 2013 Sep;30(3):163-7. PubMed

Klymenko K, Novokhatska T, Kizub I, Parshikov A, Dosenko V, Soloviev A.PKC-δ isozyme gene silencing restores vascular function in diabetic rat. J Basic Clin Physiol Pharmacol. 2014 Jan 27: PubMed

Novokhatska T, Tishkin S, Dosenko V, Boldyriev A, Ivanova I, Strielkov I, Soloviev A.Correction of vascular hypercontractility in spontaneously hypertensive rats using shRNAs-induced delta protein kinase C gene silencing. Eur J Pharmacol. 2013 Oct 15;718(1-3):401-7. doi: 10.1016/j.ejphar.2013.08.003. Epub 2013 Aug 22. PubMed

Kyrychenko VO, Nagibin VS, Tumanovska LV, Pashevin DO, Gurianova VL, Moibenko AA, Dosenko VE, Klionsky DJ. Knockdown of PSMB7 induces autophagy in cardiomyocyte cultures: possible role in endoplasmic reticulum stress.Pathobiology. 2014;81(1):8-14. PubMed

Bačová B, Radošinská J, Viczenczová C, Knezl V, Dosenko V, Beňova T, Navarová J, Gonçalvesová E, van Rooyen J, Weismann P, Slezák J, Tribulová N. Up-regulation of myocardial connexin-43 in spontaneously hypertensive rats fed red palm oil is most likely implicated in its anti-arrhythmic effects. Can J Physiol Pharmacol. 2012 Sep;90(9):1235-45. (0,857) PubMed

Benova T, Viczenczova C, Radosinska J, Bacova B, Knezl V, Dosenko V, Weismann P, Zeman M, Navarova J, Tribulova N. Melatonin attenuates hypertension-related proarrhythmic myocardial maladaptation of connexin-43 and propensity of the heart to lethal arrhythmias // Can J Physiol Pharmacol. 2013 Aug;91(8):633-9. PubMed

Dosenko VE, Gurianova VL, Surova OV, Stroy DA, Moibenko AA. Mature and immature microRNA ratios in cultured rat cardiomyocytes during anoxia-reoxygenation. Exp Clin Cardiol. 2012 Summer;17(2):84-7. (0,576) PubMed

Drevytska T, Gavenauskas B, Drozdovska S, Nosar V, Dosenko V, Mankovska I. HIF-3α mRNA expression changes in different tissues and their role in adaptation to intermittent hypoxia and physical exercise. Pathophysiology. 2012 Jun;19(3):205-14. PubMed

Klionsky Daniel J. and 1269 others Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy. 2012 Vol. 8, Issue 4 (7,453) PubMed

Klionsky, Daniel J.; Abdelmohsen, Kotb; Abe, Akihisa; et al. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) // AUTOPHAGY (2016) V. 12, # 1, P. 1-222. PubMed

Kyrychenko S, Tishkin S, Dosenko V, Ivanova I, Novokhatska T, Soloviev A. The BK(Ca) channels deficiency as a possible reason for radiation-induced vascular hypercontractility. Vascul Pharmacol. 2012 Mar;56(3-4):142-9. (1,986) PubMed

Lisovyy O.O., Dosenko V.E., Nagibin V.S., Tumanovska L.V., Korol M.O., Surova O.V., Moibenko O.O. Cardioprotective effect of 5-lipoxygenase gene (ALOX5) silencing in ischemia-reperfusion // Acta Biochim Pol. – 2009. – V. 56. – №4. – P.687-94. PubMed

Lushnikova I. Orlovsky M. Dosenko V. Maistrenko A. Skibo D. Brief anoxia preconditioning and HIF prolyl-hydroxylase inhibition enhances neuronal resistance in organotypic hippocampal slices on model of ischemic damage// Brain Res. 2011. V. 1386. P.175-183PubMed

Panasiuk O., A. Shysh, A. Bondarenko, A. Moibenko. Omega-3 PUFA-enriched diet differentially protects two subpopulations of myocardial mitochondria against Ca2+-induced injury // Experimental and Clinical Cardiology – 2013. – Vol.18, № 1. – P. e60-e64. PubMed

Pashevin DA, Tumanovska LV, Dosenko VE, Nagibin VS, Gurianova VL, Moibenko AA. Antiatherogenic effect of quercetin is mediated by proteasome inhibition in the aorta and circulating leukocytes. – Pharmacol Rep. 2011;63(4):1009-18 PubMed

Pashevin, Denis O.; Nagibin, Vasyl S.; Tumanovska, Lesya V.; et al. Proteasome Inhibition Diminishes the Formation of Neutrophil Extracellular Traps and Prevents the Death of Cardiomyocytes in Coculture with Activated Neutrophils during Anoxia-Reoxygenation // PATHOBIOLOGY (2015) V. 82, # 6, P. 290-298 PubMed

Radosinska J, Bacova B, Bernatova I, Navarova J, Zhukovska A, Shysh A, Okruhlicova L, Tribulova N. Myocardial NOS activity and connexin-43 expression in untreated and omega-3 fatty acids-treated spontaneously hypertensive and hereditary hypertriglyceridemic rats // Mol Cell Biochem. – 2011. – N.347(1-2). – P.163-73. PubMed

Voitychuk O.I., Strutynskyi R.B., Moibenko O.O., Shuba Y.M. Effects of fluorine-containing opener of ATP-sensitive potassium channels, pinacidil-derivative flocalin, on cardiac voltage-gated sodium and calcium channels // NSAP. – 2012.-V.385. -№11. – Р. 1095-1102. (2,7) PubMed

Voitychuk O.I., Strutynskyi R.B., Yagupolskii L.М., Tinker A., Moibenko O.O., Shuba Y.M. Sarcolemmal cardiac KATP channels as a target for the cardioprotective effects of the fluorine-containing pinacidil analogue flocalin // Brit J Pharmacol. – 2011.-V.162. -№3. –Р. 701-711. PubMed

Full List of our Publications

Get in touch with us

4 Bogomoletz str., Kyiv 01024, Ukraine
+38-044-256-24-81
dosenko@biph.kiev.ua

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